Making Sure You've Created a Recombinant Plasmid Why Do You Need To Verify LAB 4 Verification of Restriction and Ligation Using Gel Electrophoresis Questions Glossary
Making Sure You've Got a Recombinant Plasmid Why Do You Need To Verify Verification of the Recombinant Plasmid Using Gel Electrophoresis Questions Glossary
Getting Recombinant Plasmids Into Bacteria Transforming Bacteria with Recombinant Plasmids Transforming Bacteria with the Ligation Products Questions Glossary
Getting Recombinant Plasmids Into Bacteria Transforming Bacteria with Recombinant Plasmids LAB 5A Transforming Bacteria with the pARA-R Plasmid Questions Glossary
Getting Recombinant Plasmids into Bacteria Your Challenge Plasmids and Restriction Enzymes Producing Human Therapeutic Proteins in Bacteria Clone That Gene Transforming Bacteria with Recombinant Plasmids LAB 5B Transforming Bacteria with a Recombinant Plasmid (pARA-R) Questions Glossary
Getting What We Need Producing the Protein of Interest LAB 6 Purifying the Flourescent Protein Questions Glossary
Credits About ABE Program Introduction Program Introduction Glossary
The next steps in DNA cloning are bacterial transformation and selection. Once we have the recombinant DNA with your gene of interest, it is inserted into bacteria (transformation) and those bacteria that contain the plasmid we’re looking for are selected. We can then induce those bacteria to produce the protein we’re interested in (whether it is red fluorescent protein or a medical product such as insulin) and purify it.
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A biodome is a container for growing plants that doesn't need watering. Learn how to make one in this activity from Zoom.